| PL | Athlete | Yr. | Team | Time | Pts | HPL |
|---|---|---|---|---|---|---|
| 1 | Kelly Wycoff | SR | Fort Hays St. | 40.32 | 1 (1) | |
| 2 | Keiteyana Parks | SR | Kansas State | 40.74 | 2 (1) | |
| 3 | Sarah Horan | FR | Oklahoma State | 41.85 | 4 (1) | |
| 4 | Amber Forbes | SR | Fort Hays St. | 42.33 | 3 (1) | |
| 5 | Stephanie Ferrante | SR | Oklahoma State | 42.37 | 3 (2) | |
| 6 | Emily Hart | SR | Oklahoma State | 42.81 | 1 (2) | |
| 7 | Yanoudji Diarra | SR | Fort Hays St. | 42.99 | 5 (1) | |
| 8 | Jordan Rockins | FR | Oklahoma State | 43.31 | 2 (2) | |
| 9 | Peri Lange | SO | Fort Hays St. | 43.51 | 5 (2) | |
| 10 | Scarlett Hill | FR | Nebraska-Omaha | 43.81 | 3 (3) | |
| 11 | Gianna Moulis | SO | Oklahoma State | 44.50 | 1 (3) | |
| 12 | Bree Hysaw | FR | Fort Hays St. | 44.92 | 4 (2) | |
| 13 | Haley George | FR | Fort Hays St. | 47.02 | 5 (3) | |
| Jaycee Schettler | FR | Nebraska-Omaha | DQ | |||
| Courtney Nemechek | JR | Fort Hays St. | DQ |