| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | 121 | ROY, Juliette | Unattached | 1:05.00 | |
| 4 | 105 | MARTIN, Haley | Nanaimo & District Track & Field Club | 55.72 | |
| 5 | 92 | FORREST, Freya | Unattached | 58.90 | |
| 6 | 83 | CAMERON, Mia | Victoria Speed Project | 57.14 | |
| 7 | 130 | TIMM, Anika | Unattached British Columbia | 1:01.33 |