| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ALLSHOUSE, Emery | Denver South High School | 2:39.59 |
| 2 | OLSEN, Tessa | George Washington High School | 2:40.16 |
| 3 | BURKHOLDER, Magdalena | Denver North High School | 2:41.80 |
| 4 | RUSIN, Natalie | Denver South High School | 2:43.91 |
| 5 | VUONG, Kyra | Rangeview High School | 2:48.20 |
| 6 | CAMPOS, Soriyah | Vista Peak High School | 2:54.21 |
| 7 | RIMKUS, Chandler | Thomas Jefferson High School | 3:00.35 |
| 8 | TINOCO, Angel | Denver West High School | 3:01.80 |
| 9 | MCDANIEL, Ellery | Denver North High School | 3:02.15 |
| 10 | VILLASENOR, Alejandra | Hinkley High School | 3:05.87 |
| 11 | MARTINEZ, Iliana | Abraham Lincoln High School | 3:20.32 |
| 12 | ANSEBO, Nebiat | Far Northeast High School | NT |
| 13 | LOPEZ, Jade | Abraham Lincoln High School | NT |
| 14 | MORALES, Melany | Abraham Lincoln High School | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MUCHARSKY, Rosie | Denver East High School | 2:15.59 |
| 2 | JENULIS, Elizabeth | George Washington High School | 2:17.69 |
| 3 | ATKINSON, Giana | Northfield High School | 2:22.79 |
| 4 | TERNLUND, Melanie | Rangeview High School | 2:26.99 |
| 5 | CHOMAS, Scout | Denver East High School | 2:28.47 |
| 6 | OCHOA, Julieta | Denver East High School | 2:29.37 |
| 7 | STANSBURY, Charly | Northfield High School | 2:30.86 |
| 8 | DENNIS, Kelsey | Northfield High School | 2:33.47 |
| 9 | ROUSH, Anna | Denver South High School | 2:33.54 |
| 10 | OLBRICHT-SIMON, Leila | Thomas Jefferson High School | 2:34.46 |
| 11 | MATHER, Leah | Rangeview High School | 2:36.43 |
| 12 | KRAMER, Jenasis | Vista Peak High School | 2:37.39 |
| 13 | GRAHAM, Sophie | George Washington High School | 2:39.38 |
| 14 | SMITH, Anastasia | Vista Peak High School | 2:39.53 |