| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BARROW, Ella | J. H. Rose | 2:22.86 |
| 2 | CARSON, Carys | J. H. Rose | 2:28.43 |
| 3 | HENSLEE, Mary M | J. H. Rose | 2:30.68 |
| 4 | REVIOR, Paityn | White Oak | 2:38.00 |
| 5 | WRIGHT, Maria | J. H. Rose | 2:39.55 |
| 6 | MCCORMICK, Elena | White Oak | 2:49.00 |
| 7 | ABEL, Willow | South Central | 2:57.00 |
| 8 | OSBORNE, Ariana | South Central | 3:02.56 |
| 9 | LEWIS, Chloe | White Oak | 3:04.52 |
| 10 | OYARO, Lorine | South Central | 3:26.05 |
| 11 | MCKAY, Ella | White Oak | 3:38.00 |
| 12 | KLEBER, Tiara | White Oak | 3:39.00 |
| 13 | GARCIA, Emma | J. H. Rose | NT |
| 14 | CHUNG, Bella | White Oak | NT |
| 15 | DARANTES RODRIGUEZ, Maite | J. H. Rose | NT |
| 16 | DAVIS, Alana | South Central | NT |
| 17 | FAIL, Kannyn | Charles B. Aycock | NT |
| 18 | BROTHERS, Jayla | South Central | NT |
| 19 | HARPER, Madison | White Oak | NT |
| 20 | HAWKINS, Destiny | South Central | NT |
| 21 | HENDERSON, Alexia | South Central | NT |
| 22 | HURWITZ, Raya | South Central | NT |