| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | VINSON, Lumariah | North Edgecombe | NT |
| 3 | KELLY, Makayla | Sallie B. Howard School | 51.62 |
| 4 | MILLER, Samaria | Sallie B. Howard School | 48.05 |
| 5 | CHERRY, Morgan | Sallie B. Howard School | 48.18 |
| 6 | DICKENS, Sa' Mijah | North East Carolina Preparatory | NT |
| 7 | JACKSON, Amari | Southeast Halifax | NT |
| 8 | BATTLE, Tykaela | North Edgecombe | NT |