| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Park Ridge (Maine East) | A | Canunayon | Odantabao | Ng | Lorenc | 1:56.00 | 
| 2 | Wheeling | A | Solis-Mui | Guerrero | Pavlov | Glassberg | 1:50.00 | 
| 3 | Highland Park | A | Crawford | Liss | Rosenthal | Edelstein | 1:42.00 | 
| 4 | Arlington Heights (Hersey) | A | McKenzie | Ibe | Farrell | Alonso | 1:38.20 | 
| 5 | Barrington | A | Marian | Lewandowski | Wright | Hall | 1:37.20 | 
| 6 | Mt. Prospect (Prospect) | A | Hanson | Calhoun | Lawlor | Numani | NT | 
| 7 | Huntley | A | Costa | Giron | Tepper | Laughlin | 1:40.00 | 
| 8 | Des Plaines (Maine West) | A | Martinez | Zheng | Marinho | Hauptman | 1:45.00 | 
| 9 | Skokie (Niles North) | A | Andrews | Jeanpois | Robinson Jr. | Osinowo | 1:51.40 |