| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Des Plaines (Maine West) | A | Ferrariolo | Adcock | Hornish | Servin | 1:55.00 | 
| 2 | Wheeling | A | Ghibea | Campos | Plazola | Carreno | 1:50.00 | 
| 3 | Park Ridge (Maine East) | A | Irving | Rotter | Fakorede | Munkhtur | 1:42.20 | 
| 4 | Huntley | A | Van De Voorde | Giuseffi | Oruche | Bower | 1:37.50 | 
| 5 | Mt. Prospect (Prospect) | A | Carlucci | Vaeth | Bednarski | Orlando | 1:35.00 | 
| 6 | Barrington | A | Thorson | Matiyow | Marin | Matarieh | 1:36.90 | 
| 7 | Arlington Heights (Hersey) | A | Jenkins | Casey | Fung | Cohen | 1:38.20 | 
| 8 | Highland Park | A | Knapp | Nunez | Rojas | Huicochea | 1:43.00 | 
| 9 | Skokie (Niles North) | A | Lazar | Infantes | Sather | Quiros | 1:51.10 |