| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Des Plaines (Maine West) | A | Amar | Adcock | Hornish | Servin | 50.00 | 
| 2 | Skokie (Niles North) | A | Sather | Infantes | Akinpelu | Quiros | 48.30 | 
| 3 | Highland Park | A | DePaolo | Nunez | Rojas | Knapp | 47.50 | 
| 4 | Arlington Heights (Hersey) | A | Jenkins | Casey | Fung | Cohen | 46.50 | 
| 5 | Mt. Prospect (Prospect) | A | Easter | Cichy | Bednarski | Orlando | 45.10 | 
| 6 | Barrington | A | Jackson | Marin | Matarieh | Thorson | 46.34 | 
| 7 | Huntley | A | Van De Voorde | Giuseffi | Oruche | Bower | 46.50 | 
| 8 | Park Ridge (Maine East) | A | Fakorede | Rotter | Munkhtur | Irving | 47.61 | 
| 9 | Wheeling | A | Bustos | Porada | Campos | Ghibea | 49.50 |