| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | CHU, Tiffany | JR | Hoffman Estates (Conant) | 2:55.13 | |
| 2 | AVDJIEV, Yoanna | SO | Rolling Meadows | 3:01.70 | |
| 3 | PATEL, Jeeya | FR | Hoffman Estates (Conant) | 3:03.10 | |
| 4 | DAVIS, Jennifer | SO | Rolling Meadows | 3:07.50 | |
| 5 | LUSZCEK, Olivia | SO | Hoffman Estates (Conant) | 3:39.10 | |
| 6 | ANTUNA, Mia | SO | Hoffman Estates (Conant) | 3:39.20 | |
| 7 | DOHNAL, Rachel | SO | Hoffman Estates (Conant) | 3:39.70 | |
| 8 | KAEGI, Sophie | JR | Palatine (H.S.) | NT | |
| 9 | RAMIREZ, Cristina | JR | Palatine (H.S.) | NT |