| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | WIKAR, Mia | SO | Palatine (H.S.) | 1:02.64 | |
| 2 | HOLLIS, Kaylee | JR | Palatine (H.S.) | 59.86 | |
| 3 | CHACKO, Shreya | JR | Rolling Meadows | 58.00 | |
| 4 | OSTROWSKI, Eden | FR | Hoffman Estates (Conant) | 54.64 | |
| 5 | MOORE, Linna | SO | Palatine (H.S.) | 56.64 | |
| 6 | PATEL, Isha | FR | Hoffman Estates (Conant) | 59.80 | |
| 7 | WOZNY, Trinity | SO | Rolling Meadows | 1:00.40 | |
| 8 | PALACIOS, Mia | SO | Palatine (H.S.) | 1:04.04 |