| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 3 | DZIEZA, Amelia | 15-16 | Palatine Pack TFCC | 33.24 | 
| 4 | RICE, Emma | 15-16 | Palatine Pack TFCC | 30.56 | 
| 5 | PEAIRS, Addisyn | 15-16 | Palatine Pack TFCC | 31.64 | 
| 6 | BUTLER, Pryncess | SO | Unattached | 35.55 | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | SALOM HAMWI, Giselle | JR | Unattached | 28.70 | 
| 2 | LETZIG, Emma | SR | Unattached | 28.00 | 
| 3 | JARRETT, London | 13-14 | Legacy Runners Athletic Club | 26.79 | 
| 4 | PAUL, Elaine | JR | Unattached | 26.40 | 
| 5 | PRATT, Katelyn | SR | Unattached | 26.60 | 
| 6 | BASCO, Caitlin | 15-16 | Palatine Pack TFCC | 27.20 | 
| 7 | CHILDRESS, Diaymond | Legacy Runners Athletic Club | 28.28 | |
| 8 | GINSBERG, Audrey | SR | Unattached | 29.00 |