| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KMAK, Gianna | SR | Chicago (Mother McAuley) | NT | |
| 2 | DEACY, Nora | SR | Chicago (Mother McAuley) | NT | |
| 3 | KARCZEWSKI, Emma | SR | Chicago (Marist) | 1:04.90 | |
| 4 | MCNICHOLAS, Nora | SR | Chicago (Marist) | 1:07.50 | |
| 5 | COAKLEY, Lillian | JR | Chicago (Mother McAuley) | NT | |
| 6 | JONES, Jada | FR | Chicago (Mother McAuley) | NT |