| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Minooka | A | Moody | Kueltzo | Richardson | McPherson | NT | 
| 3 | Wilmette (Loyola Academy) | A | Akela | Monterola | Tamas | Hysenukaj | 1:30.00 | 
| 4 | Mt. Prospect (Prospect) | A | DeLeon | Medina | Medina | Wind | 1:03.00 | 
| 5 | Glenview (Glenbrook South) | A | Al-Twal | des Groseilliers | Hirsch | Karavites | NT | 
| 6 | Rockton (Hononegah) | A | Pierson | Molander | Clarke | Harris | 1:05.00 | 
| 7 | Belvidere (North) | A | Hulstedt | Downey | Andrist | VanFleet | DNS | 
| 8 | Gurnee (Warren) | A | Garcia | Garcia-Lewis | Copenharve | Dixon | NT | 
| 9 | Naperville (Central) | A | Villanova | Roozeboom | Small | Schrom | NT |