| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | North Charleston | A | Blakeney | Clement Jr | Sparks | Holley | NT |
| 4 | Fort Dorchester | A | 1:14.09 | ||||
| 5 | Pinewood Prep | A | Bellush | Heath | Wiggins | Stallworth | 45.48 |
| 6 | Oceanside Collegiate Academy | A | Harriott | Baldwin | Feinzig | Hedgepath | 47.43 |
| 7 | Waccamaw | A | Grate | Grate | Rogers | Skinner | NT |
| 8 | West Ashley | A | Downing | Dolloff | Gadsden | Holman | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Woodland | A | Farray | Purvis | Majette | Pinckney | 45.28 |
| 2 | Lucy Beckham | A | Fletcher | Tolley | Ombres | Walton | 44.81 |
| 3 | Cane Bay | A | Houpe | Espinal | Green | Johnson | 43.73 |
| 4 | Summerville | A | Lailson | Pettiford | Deweese | Washington | 42.98 |
| 5 | Philip Simmons | A | Porcher | Steed | Sullivan | Asbury | 42.78 |
| 6 | Ashley Ridge | A | Jefferson | Austin | Deweese | Salley | 42.91 |
| 7 | Wando | A | 43.4h | ||||
| 8 | Stall | A | Davis | Gordon | Bacon | Foster | 44.8h |
| 9 | Berkeley | A | mack | Thompson | Murray | Nelson | 45.02 |