| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Smith, Payton | Avon Grove | 47.59 |
| 2 | Hearn, Ryleigh | Downingtown West | 51.70 |
| 3 | Compher, Emma | Downingtown West | 51.93 |
| 4 | Caronia, Alex | West Chester Henderson | 53.91 |
| 5 | Jennings-Scott, Giovanna | Downingtown East | 54.03 |
| 6 | Hauer, Carly | Downingtown East | 54.31 |
| 7 | Sujesh, Keerthana | Downingtown East | 55.77 |
| 8 | Keefe, Maeve | West Chester East | 56.83 |
| 9 | Montgomery, Alexis | Downingtown West | 56.86 |
| 10 | Minchoff, Lucy | Downingtown West | 56.95 |
| 11 | Uhlman, Deborah | Bishop Shanahan | 57.35 |
| 12 | Shaefer, Reilly | Downingtown East | 57.36 |
| 13 | Nolan, Zoe | West Chester Henderson | 57.56 |
| 14 | Brunschwyler, Charlotte | Downingtown West | 57.90 |
| 15 | Davies, Grace | Downingtown East | 58.38 |
| 16 | van der Steur, Anastasia | Bishop Shanahan | 58.61 |
| 17 | Yarnall-Stevenson, Charlotte | Downingtown East | 1:00.01 |
| 18 | Bousquet, Sophie | Downingtown East | 1:00.05 |
| 19 | Carlow, Ana | West Chester Henderson | 1:00.88 |
| 20 | Robinson, Julia | Downingtown East | 1:03.76 |
| 21 | Rogelstad, Sarah | Bishop Shanahan | 1:03.97 |
| 22 | Conrad, Caroline | West Chester Henderson | 1:07.94 |
| 23 | Fuhr, Mya | Downingtown West | NT |