| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | Stanfield, Chloe | JR | D-West | 48.18 |
| 2 | Kline, Erin | JR | Haverford Township HS | 48.66 |
| 3 | Smith, Denique | SR | Upper Darby | 49.10 |
| 4 | Greiner, Alyssa | 9 | OJR | 50.09 |
| 5 | Touring, Evie | JR | WC East | 50.18 |
| 6 | Forrest, Julia | 9 | OJR | 50.35 |
| 7 | Kora, Jabou | SR | Upper Darby | 51.00 |
| 8 | Metzger, Allyson | SO | VMA | 51.35 |
| 9 | Madyun, Inaya | SR | Henderson | 52.43 |
| 10 | Davis, Kelly | JR | D-West | 52.57 |
| 11 | Curran, Karson | SR | Padua Academy | 53.14 |
| 12 | Desrosiers, Bailey | 9 | Henderson | 53.44 |
| 13 | Mummert, Hannah | JR | Rustin | 54.04 |
| 14 | Becker, Cassidy | 9 | Padua Academy | 54.97 |
| 15 | Philador, Tamara | SO | Upper Darby | 55.13 |
| 16 | Rovinsky, Isabel | SO | Germantown Friends School | 55.46 |
| 17 | Minchoff, Lucy | SO | D-West | 56.95 |
| 18 | Potter, Julie | SO | Haverford Township HS | 57.35 |
| 19 | Kelch, Nikki | 9 | D-East | 57.79 |
| 20 | Porta, Kaitlyn | SO | VMA | 58.38 |
| 21 | Porta, Ali | 9 | VMA | 58.43 |
| 22 | Frascone, Juliana | 9 | Spring Ford | 59.73 |
| 23 | Plunkett, Mairead | 9 | Haverford Township HS | 59.92 |
| 24 | Diguiseppe, Emma | 9 | Henderson | 1:00.31 |
| 25 | Moutchia, Akene | 9 | Spring Ford | 1:00.74 |
| 26 | Wise, Ava | 9 | D-East | 1:03.35 |
| 27 | Swinton, Allison | 9 | D-East | 1:05.14 |