| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Upper Darby | A | 49.37 | ||||
| 2 | D-West | A | Alston, Jada | DiOttavio, Lauren | Tucker, Maya | Jones, Audrey | 50.44 |
| 3 | Henderson | A | 51.20 | ||||
| 4 | Upper Darby | B | 51.90 | ||||
| 5 | Germantown Friends School | A | Abdus-Salaam, Zuri | Osborne, Lola | Milbourne, Lauryn | Vincent, Asha | 51.96 |
| 6 | D-East | A | McDonald, Caroline | Norman, Aisha | Sweeney, Niamh | Binns, Olivia | 52.12 |
| 7 | Garnet Valley | A | 52.61 | ||||
| 8 | Garnet Valley | B | 53.10 | ||||
| 9 | D-West | B | Jahromi, Lili | Kohute, Sierra | Riggs, Hannah | Lubwama, Diana | 54.39 |
| 10 | Henderson | B | 54.39 | ||||
| 11 | WC East | A | Leeds, Giovina | Diasseny, Shakria | Pickerd, Delaney | Moneme, Chinonye | 54.51 |
| 12 | Spring Ford | A | Grubb, Amanda | Grant, Kaela | Hang, Lana | Woods, Katharina | 55.00 |
| 13 | Padua Academy | A | Brown, Adeline | Hays, Peyton | Flanagan, Maggie | Fajinmi, Tolu | 56.70 |