| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Octorara | A | Raison-Peters, Zion | Williams, Isabelle | Jordan, Mary | Outland, Jesse | 1:00.00 |
| 4 | Fred S. Engle Middle School | A | 56.00 | ||||
| 5 | Chichester Middle School | A | 58.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Lionville Middle School | A | Vadher, Jaanvi | Wolf, Jack | Sardella, Anna | Carcella, Tyler | 54.20 |
| 3 | Kennett Middle School | A | 53.50 | ||||
| 4 | Penn Wood Middle School | A | 50.30 | ||||
| 5 | Stetson Middle School | A | Stocardo, Nick | Stocardo, Dominic | Stephney, Kayla | Darwon, Keturah | 52.00 |
| 6 | Great Valley Middle School | A | 54.00 | ||||
| 7 | Peirce Middle School | A | Bell, Destiny | Caronia, Alexandra | Garcia, Danny | Lubeski, Preston | 54.50 |