| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Pash, Calvin | West Chester Henderson | 40.60 |
| 2 | Zurlo, John | West Chester Henderson | 41.20 |
| 3 | Washington, Nick | West Chester Henderson | 41.50 |
| 4 | Davidson, Jack | West Chester Henderson | 42.10 |
| 5 | Phung, Ben | West Chester Henderson | 42.20 |
| 6 | Pron, Carson | West Chester Henderson | 42.20 |
| 7 | Trice, Ryan | West Chester Henderson | 42.20 |
| 8 | Chilet, Alex | West Chester Henderson | 42.20 |
| 9 | McCormack, Cole | West Chester Henderson | 43.10 |
| 10 | Conrad, Trey | West Chester Henderson | 43.30 |
| 11 | Murvala, Siddhu | West Chester Henderson | 44.10 |
| 12 | Parkinson, Chase | West Chester Henderson | 44.30 |
| 13 | Mahesh, Aku | West Chester Henderson | 44.60 |
| 14 | Romano, Alex | West Chester Henderson | 44.80 |
| 15 | Laurento, Logan | West Chester Henderson | 45.60 |
| 16 | DeFonzo, Ben | West Chester Henderson | 46.50 |
| 17 | Fuguet, Sam | West Chester Henderson | 46.70 |
| 18 | Axenfeld, Ben | West Chester Henderson | 47.10 |
| 19 | Kress, Ethan | West Chester Henderson | 48.90 |
| 20 | Farrell, Jake | Downingtown West | 51.66 |
| 21 | Hyka, Erik | West Chester Henderson | 52.10 |
| 22 | Debuque, Henry | Downingtown West | 52.14 |
| 23 | Bulger, Nate | Downingtown West | 52.98 |
| 24 | Zouras, Dominic | Downingtown West | 53.45 |
| 25 | Barron, Josh | Downingtown West | 55.00 |
| 26 | Florkowski, Andrew | Downingtown West | 56.00 |
| 27 | Parham, Tony | Downingtown West | 56.00 |
| 28 | Torres-Mercado, Ian | Downingtown West | 57.99 |
| 29 | Williams, Xavion | Downingtown West | 58.00 |
| 30 | Zou, Andrew | Downingtown West | 1:00.13 |
| 31 | Dean, Stephen | Downingtown West | 1:00.14 |
| 32 | Czap, Connor | Downingtown West | 1:00.54 |
| 33 | Guntupalli, Bhargav | Downingtown West | 1:02.33 |