| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Mattia, Morgan | Twin Valley | 47.24 |
| 2 | Acinapura, Annabella | Twin Valley | 48.24 |
| 3 | Lerch, Emily | Twin Valley | 48.32 |
| 4 | Richards, Natalie | West Chester Henderson | 49.20 |
| 5 | Lister, Allie | West Chester Henderson | 49.50 |
| 6 | Scherrer, Liesl | West Chester Henderson | 49.50 |
| 7 | Beatty, Rachel | West Chester Henderson | 51.20 |
| 8 | Mulhern, Jenna | West Chester Henderson | 51.20 |
| 9 | Mattera, Gina | Twin Valley | 52.24 |
| 10 | Gardner, Katelyn | Twin Valley | 53.45 |
| 11 | Gehron, Amber | West Chester Henderson | 54.30 |
| 12 | Quigley, Kylie | West Chester Henderson | 54.80 |
| 13 | Bradley, Kayla | West Chester Henderson | 55.20 |
| 14 | Elduff, Sabrina | West Chester Henderson | 55.40 |
| 15 | Hammond, Emika | West Chester Henderson | 55.40 |
| 16 | Ng, Chloe | West Chester Henderson | 55.40 |
| 17 | Addy, Morgan | Twin Valley | 55.64 |
| 18 | Newdeck, Emily | West Chester Henderson | 55.80 |
| 19 | Doerr, Kiely | West Chester Henderson | 56.80 |
| 20 | Mallozzi, Brie | West Chester Henderson | 58.70 |
| 21 | Walsh, Brielle | West Chester Henderson | 59.70 |
| 22 | Boorujy, Ashley | West Chester Henderson | 59.90 |
| 23 | Jarman, Calista | Downingtown West | 1:00.00 |
| 24 | Tennant, Grace | Twin Valley | 1:00.34 |
| 25 | Compher, Emma | Downingtown West | 1:01.00 |
| 26 | Zeblium, Lili | Downingtown West | 1:01.87 |
| 27 | Duffy, Elise | Downingtown West | 1:02.14 |
| 28 | Grafje, Kylie | Downingtown West | 1:02.45 |
| 29 | Singh, Arushi | West Chester Henderson | 1:04.50 |
| 30 | Suchecki, Adelaide | Downingtown West | NT |
| 31 | Reason, Kiley | Twin Valley | NT |
| 32 | Reason, Kiley | Twin Valley | NT |
| 33 | Sharp, Kathryn | Twin Valley | NT |
| 34 | Sharp, Kathryn | Twin Valley | NT |