| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Bejarano, Summer | Unattached | 5:11.73 |
| 2 | Piombino, Sonia | WC East TC | 5:13.02 |
| 3 | Tarpley, Carolyn | Downingtown West TC | 5:18.04 |
| 4 | Jappe, Olivia | Unattached | 5:19.00 |
| 5 | Piccone, Sofia | WC Rustin TC | 5:22.00 |
| 6 | Kimmel, Madison | Unattached | 5:25.00 |
| 7 | Martin, Anna | Unattached | 5:25.00 |
| 8 | montini, olivia | Unattached | 5:25.00 |
| 9 | Scherrer, Liesl | WC Henderson TC | 5:25.04 |
| 10 | Paci, Claire | Unattached | 5:25.74 |
| 11 | Macdonald, Anna | GFS TC | 5:27.78 |
| 12 | Kelly, Amanda | WC Henderson TC | 5:28.63 |
| 13 | Montini, Olivia | Unattached | 5:30.00 |
| 14 | Flores, Francie | Unattached | 5:30.01 |
| 15 | Bell, Caroline | Unattached | 5:36.53 |
| 16 | Henisz, Katya | GFS TC | 5:37.36 |
| 17 | Greco, Isabella | Unattached | 5:37.75 |
| 18 | Marion, Abby | Unattached | 5:39.00 |
| 19 | Rochon, Taylor | Downingtown West TC | 5:40.55 |
| 20 | Miller, Caroline | WC Henderson TC | 5:42.89 |
| 21 | Flynn, Carissa | Unattached | 5:44.19 |
| 22 | Holmes, Grace | Unattached | 5:45.00 |
| 23 | Marcinkowski, Lexie | Unattached | 5:45.00 |
| 24 | Posh, Laura | Twin Valley TC | 5:45.65 |
| 25 | Ambrosine, Brianna | WC Rustin TC | 5:48.00 |
| 26 | O'Hair, Molly | WC Rustin TC | 5:48.00 |
| 27 | Puckett, Hannah | Penncrest TC | 5:48.00 |
| 28 | Bender, Jordan | WC Henderson TC | 5:51.48 |
| 29 | Bevec, Kate | Great Valley TC | 5:52.74 |
| 30 | Thatcher, Abigail | Twin Valley TC | 5:53.69 |
| 31 | Bell, Riley | Northeastern TC | 5:55.00 |
| 32 | Kurilla, Haylie | Northeastern TC | 5:55.00 |
| 33 | Kurilla, Rylie | Northeastern TC | 5:55.00 |
| 34 | Olsen, Lucy | WC Henderson TC | 5:58.22 |
| 35 | Bender, Riley | WC Henderson TC | 6:00.00 |
| 36 | Oskison, Paige | Downingtown West TC | 6:07.24 |
| 37 | Kyle, Nora | Bishop Shanahan TC | 6:08.22 |
| 38 | Ward, Jessica | Bishop Shanahan TC | 6:10.00 |
| 39 | Loeper, Penelope | Bishop Shanahan TC | 6:12.96 |
| 40 | Albanese, Noelle | Great Valley TC | 6:15.09 |
| 41 | Maloney, Emily | Downingtown West TC | 6:15.23 |
| 42 | Quitel, Kira | GFS TC | 6:15.50 |
| 43 | Greco, Evanthia | Penncrest TC | 6:27.00 |
| 44 | Ward, Kirsten | Bishop Shanahan TC | 6:30.00 |
| 45 | Rittenhouse, Julianna | Anchor Christian TC | 6:30.00 |
| 46 | Moser, Keira | Downingtown East TC | 6:30.24 |
| 47 | Morrin, Keira | Downingtown East TC | 6:32.24 |
| 48 | Delucca, Andrea | Downingtown East TC | 6:42.24 |
| 49 | Colson, Page | WC Henderson TC | 7:09.31 |
| 50 | Dutton, Paige | WC Rustin TC | 7:19.00 |