| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Boone, Tyler | Roman Catholic TC | 49.97 |
| 2 | Fitzgerald, Carter | Unattached | 52.26 |
| 3 | Kravitz, Colin | Unattached | 52.76 |
| 4 | Lubeski, Preston | WC Henderson TC | 52.82 |
| 5 | Newell, Aaron | WC Henderson TC | 53.10 |
| 6 | Conzelmann, JJ | Downingtown West TC | 53.11 |
| 7 | Kozel, Daniel | Avon Grove TC | 53.84 |
| 8 | Ruark, Connor | Avon Grove TC | 53.95 |
| 9 | Bandari, Agasthya | Great Valley TC | 54.32 |
| 10 | Zielinski, Jake | Great Valley TC | 54.35 |
| 11 | Robson, Donald | Downingtown East TC | 54.61 |
| 12 | Dixon, Jacob | Downingtown West TC | 54.75 |
| 13 | Dever, Aiden | Downingtown East TC | 54.80 |
| 14 | Lennon, Noah | Avon Grove TC | 54.90 |
| 15 | DeSantis, Brendan | Great Valley TC | 54.90 |
| 16 | Nolan, Pierce | Great Valley TC | 55.50 |
| 17 | Denlinger, Tyler | Unattached | 55.50 |
| 18 | Vitale, Drake | Great Valley TC | 55.52 |
| 19 | Kearney, Jak | Roman Catholic TC | 55.64 |
| 20 | Langan, Aidan | WC Henderson TC | 56.04 |
| 21 | DiFonzo, Neil | WC East TC | 56.09 |
| 22 | Goldfarb, Connor | LaSalle College HSTC | 56.30 |
| 23 | McCullough, Rhyland | Downingtown West TC | 56.39 |
| 24 | Potter, Will | WC Henderson TC | 56.70 |
| 25 | Harris-Thompson, Adon | Jenkintown TC | 56.97 |
| 26 | Wang, William | Anchor Christian TC | 57.00 |
| 27 | Jeffery, Luke | Kennett TC | 57.24 |
| 28 | Briger, Oliver | GFS TC | 57.50 |
| 29 | Thompson-Harris, Alon | Jenkintown TC | 57.87 |
| 30 | DeVries, Cooper | WC Henderson TC | 58.22 |
| 31 | Mixon, Isaac | Jenkintown TC | 58.46 |
| 32 | Blake, Sully | Jenkintown TC | 58.90 |
| 33 | Bartilomo, Austin | Downingtown West TC | 58.94 |
| 34 | Schetroma, Vincent | Downingtown West TC | 59.00 |
| 35 | Bergin, Matthew | WC East TC | 59.49 |
| 36 | Goines, Ashura | WC East TC | 1:00.01 |
| 37 | Pron, Carson | WC Henderson TC | 1:00.33 |
| 38 | Ridenour, Stephen | Jenkintown TC | 1:01.00 |
| 39 | Gossett, Geordan | Jenkintown TC | 1:01.25 |
| 40 | Purtle, Finn | OJR TC | 1:01.50 |
| 41 | Cattie, Jack | Upper Moreland TC | 1:01.50 |
| 42 | Kinniry, Tim | OJR TC | 1:01.58 |
| 43 | Morrison, Sean | WC Henderson TC | 1:02.06 |
| 44 | Dill, Jordan | Jenkintown TC | 1:05.51 |
| 45 | Morello, Andrew | Downingtown West TC | 1:06.72 |
| 46 | Facciolo, Angelo | Downingtown West TC | 1:07.39 |
| 47 | Lukashchuk, Andrew | ARTC | NT |