| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Pottsgrove | A | Bellamy | Jones | Smythe | Strain | 53.33 | 
| 2 | Owen J. Roberts | A | Hannevig | Larsen | Bold | Spencer | 51.16 | 
| 3 | Boyertown | A | 50.19 | ||||
| 4 | Methacton | A | Dunham | Gormley | Wang | Mellow | 50.75 | 
| 5 | Norristown | A | 52.43 |