| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | Wamsher, Makenzie | 9 | Cedar Crest | 50.00 |
| 2 | Temple, Isabella | 9 | Palmyra | 50.44 |
| 3 | McConaghy, Carmen | JR | Palmyra | 50.96 |
| 4 | Miller, Sara | JR | Eastern Lebanon County | 51.10 |
| 5 | Wamsher, Hayden | 9 | Cedar Crest | 51.21 |
| 6 | Boyer, Toccoa | SR | Northern Lebanon | 51.45 |
| 7 | Etherson, Bre | SR | Eastern Lebanon County | 52.70 |
| 8 | Missimer, Kya | SO | Annville-Cleona | 54.30 |
| 9 | Fortuna Aracena, Anabel | SR | Lebanon | 54.80 |
| 10 | Rivera Colon, Dimaris | JR | Lebanon | 55.70 |
| 11 | Davis, Andreanna | SO | Northern Lebanon | 1:02.00 |