| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Miller, Sara | Eastern Lebanon County | 50.20 |
| 2 | Hentz, Jacey | Eastern Lebanon County | 52.01 |
| 3 | McConaghy, Carmen | Palmyra | 52.20 |
| 4 | Haggerty, Mariah | Cedar Crest | 52.26 |
| 5 | Thomas, Lily | Cedar Crest | 53.46 |
| 6 | Boyer, Toccoa | Northern Lebanon | 53.86 |
| 7 | Karoscik, Neveah | Lebanon | 54.24 |
| 8 | Halbleib, Cali | Palmyra | 54.30 |
| 9 | Rank, Morgan | Northern Lebanon | 55.15 |
| 10 | Johnson, Molly | Annville-Cleona | 56.74 |
| 11 | Espada, Mya | Annville-Cleona | 57.10 |
| 12 | Rivera Colon, Dimaris | Lebanon | 1:00.00 |