| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Henderson | A | 14:30.00 | ||||
| 2 | Unionville | A | O'Leary, Erin | Poe, Rachel | Torrey, Ashley | Mazur, Erin | 14:40.00 |
| 3 | WC East | A | Andiario, Rachel | Chermak, Carly | Blanck, Ellie | Prieto, Elizabeth | 15:00.24 |
| 4 | Rustin | A | Senthil, Kaaviya | Lutcavage, Lexy | Ye, Elizabeth | Bonass, Maeve | 15:25.66 |
| 5 | Kennett | A | 16:30.00 | ||||
| 6 | Oxford Area | A | NT | ||||
| 7 | D-West | A | Grafje, Kylie | Leamy, Shea | Defibaugh, Stella | Schwarz, Tara | NT |