| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | WC East | A | Baschoff, Emma | Lewis, Danielle | Rehrig, Riley | Richardson, Reilly | 4:17.56 |
| 2 | Unionville | A | O'Leary, Erin | Beaudet, Mia | Donohue, Drew | Mandl, Cora | 4:40.00 |
| 3 | Kennett | A | 5:00.00 | ||||
| 4 | D-West | A | 5:00.24 | ||||
| 5 | Henderson | A | 5:06.94 |