| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Oxford Area | A | 1:35.00 | ||||
| 2 | D-West | A | Barry, John | Jordan, Walka | Burton, Ryan | Williams, Zavion | 1:42.00 |
| 3 | WC East | A | Zimmerman, Brennan | DiFonzo, Neil | Niemeyer, Matthew | Lombardi, Nathan | 1:42.28 |
| 4 | Great Valley | A | 1:42.83 | ||||
| 5 | Great Valley | B | 1:42.83 | ||||
| 6 | Henderson | A | Pron, Carson | 1:44.40 | |||
| 7 | Henderson | B | Boeckle, Brennan | Cohen, Michael | Hicks, Bobby | Langan, Aidan | 1:44.70 |
| 8 | Oxford Area | B | 1:45.00 | ||||
| 9 | Rustin | A | 1:46.24 | ||||
| 10 | WC East | B | Cichanowsky, Michael | Briggs, Shane | McCormick, Ryan | Boutin, Riley | 1:49.00 |
| 11 | Rustin | B | 1:55.00 | ||||
| 12 | Unionville | A | Lee, Ryan | Martin, Ben | Harlan, Ben | Trojanowski, Tanner | 1:56.00 |
| 13 | Unionville | B | Otieno, Sam | Giacoio, Alex | Ladd, Conor | Hjaltson, Simon | 2:00.00 |
| 14 | Kennett | A | 2:00.00 | ||||
| 15 | Kennett | B | 2:10.00 | ||||
| 16 | D-West | B | Michel, Chris | Madanat, Andrew | Zednik, Cooper | Harris, Nashir | NT |