| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Wunderlich, Jane | Oxford Area | 54.45 |
| 2 | Rutherford, Serena | Great Valley | 55.20 |
| 3 | Loeper, Penelope | Bishop Shanahan | 55.28 |
| 4 | Finkbiner, Ashley | Downingtown West | 55.58 |
| 5 | Jiang, Grace | Unionville | 56.68 |
| 6 | Becthold, Claudia | Kennett | 57.54 |
| 7 | Rogelstad, Sarah | Downingtown West | 57.73 |
| 8 | Klara, Olivia | West Chester Henderson | 58.14 |
| 9 | Skomorucha, Leah | West Chester Henderson | 58.16 |
| 10 | Blake, Meghan | Downingtown East | 58.34 |
| 11 | Lueders, Mackenzie | Downingtown East | 58.74 |
| 12 | Peterson, Norah | Kennett | 58.90 |
| 13 | Gray, Leah | Avon Grove | 59.00 |
| 14 | Barlow, Alexis | Kennett | 1:00.71 |
| 15 | Conrad, Caroline | West Chester Henderson | 1:05.49 |
| 16 | Richardson, Maggie | Unionville | NT |