| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | WC East | A | 3:21.00 | ||||
| 2 | Perkiomen Valley | A | 3:28.00 | ||||
| 3 | Warwick | A | 3:28.00 | ||||
| 4 | D-East | A | DeLone, Zach | Robson, Donald | Ponna, Anish | Hennessy, Joseph | 3:28.77 |
| 5 | Twin Valley | A | 3:29.50 | ||||
| 6 | Methacton | A | Balek, Andrew | Layos, Jordan | Gervais, Anthony | Clark, Alex | 3:33.22 |
| 7 | Friends' Central | A | Loudini, Zakaria | Ellison, Ryan | Veith, Alex | Thomas, Noah M | 3:33.31 |
| 8 | D-West | A | Haider, Ali | Difrancesco, Mike | Ford, Elijah | Marasco, Joey | 3:35.78 |
| 9 | Henderson | A | 3:36.52 | ||||
| 10 | OJR | A | 3:36.73 | ||||
| 11 | Upper Merion | A | 3:37.79 | ||||
| 12 | Polytech High School | A | Sabanayagam, Ty | Mack, Cameron | Capezio, Sean | Bailor, David | 3:38.34 |
| 13 | Hershey | A | 3:39.16 | ||||
| 14 | Schuylkill Valley | A | Arnold, Wolf | Gensure, Aidan | Haupt, Graham | Seymour, Luke | 3:43.10 |