| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Warwick | A | 47.25 | ||||
| 2 | Conestoga Valley | A | Castronova, Sarah | Livingston, Jane | Wentz, Dana | Hock, Jordyn | 48.08 |
| 3 | Methacton | A | Keenan, Juliana | Dunham, Camille | Dunham, Coco | Evans, Katelyn | 49.76 |
| 4 | Twin Valley | A | 50.02 | ||||
| 5 | Unionville | A | Bucci, Makayla | Hart, Meghan | Lin, Orenda | McCarthy, Lynsey | 50.40 |
| 6 | Spring Ford | A | Higgins, Emily | Lanzisera, Claire | Rawley, Devin | Scanlan, Kyra Jones | 50.48 |
| 7 | Rustin | A | 50.50 | ||||
| 8 | Friends' Central | A | Stovall, Bailie | MacFarlane, Izzy | Burd, Julia | Garret, Maya | 50.56 |
| 9 | Red Lion | A | Godfrey, Jayla | Patterson, Camree | Rowe, Julia | Beaverson, Sydney | 50.64 |
| 10 | J.P. McCaskey | A | 50.67 | ||||
| 11 | Wilson (3) | A | Trymbiski, Ednabel | Tony, Amanda | Herb, Madi | Barry, Fiona | 50.75 |
| 12 | Bishop Shanahan | A | 50.85 | ||||
| 13 | Souderton Area | A | 51.10 | ||||
| 14 | Avon Grove | A | Heckman, Olivia | O'Connor, Molly | Cousin, Kaylin | Jacquet, Ellie | 51.16 |
| 15 | OJR | A | Hannevig, Ashley | Mayk, Michelle | Kqira, Olivia | Bold, Caleigh | 51.29 |
| 16 | Padua Academy | A | 51.30 | ||||
| 17 | CD East | A | Colbert, Taja | Wallace, Markya | Curtis, Gabrielle | Bellamy-Reid, Trinity | 51.53 |
| 18 | Henderson | A | 51.69 | ||||
| 19 | Bay Shore | A | 51.69 | ||||
| 20 | Penn Manor | A | Morales, Chantal | Martinez, Saralyn | Barton, Isabelle | Miller, Jasmine | 51.79 |
| 21 | D-East | A | McDonald, Caroline | Norman, Aisha | Sweeney, Niamh | Binns, Olivia | 51.84 |
| 22 | Dover High School | A | Stedman, Nya | Toussaint, Stephanie | Garcia, Christina | Edwards, Emoni | 51.97 |
| 23 | Phoenixville Area | A | 52.00 | ||||
| 24 | ELCO | A | Nelson, Julia | Rosengrant, Devon | Rueppel, Kaitlyn | Swingholm, Natalie | 52.08 |
| 25 | CB West | A | 52.24 | ||||
| 26 | Muhlenberg | A | Simeon, Vanessa | Sierra, Jineylis | Hernanez, Julyssa | Greene, Laneya | 52.31 |
| 27 | WC East | A | Leeds, Giovina | Diasseny, Shakria | Pickerd, Delaney | Moneme, Chinonye | 52.31 |
| 28 | Polytech High School | A | Gray, Danielle | Sizer, Amiyah | Beckett, Tasiaja | Washington, Ani | 53.00 |
| 29 | Perkiomen Valley | A | 53.24 | ||||
| 30 | Upper Merion | A | 53.27 | ||||
| 31 | Washington Twp HS | A | Berndlmaier, Avarie | Smith, Megan | Repousis, Izabela | Kelly, Veronica | 53.64 |
| 32 | Mechanicsburg Area High School | A | Shreves, Lucy | Lebo, Kerrigan | Gilliard, Bella | England, Noelle | 53.74 |
| 33 | Schuylkill Valley | A | Lisa, Samantha | Searfoss, Abigall | Starr, Emily | Gilbert, Sarah | 53.99 |
| 34 | Dock Mennonite Academy | A | Landis, Kate | Kratz, Emma | Scialanca, Peyton | Mercure, Olivia | 54.20 |
| 35 | D-West | A | Devlin, Kathleen | Jahromi, Lili | Lubwama, Diana | Kiefel, Jocelyn | 54.60 |
| 36 | Delcastle High School | A | Jayla, Pulliam | Ward, Jaiden | Jefferson, Kira | Johnson, Amira | 55.00 |