| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Bishop Shanahan | B | Houser, Lauren | Egan, Avery | Nealy, Elizabeth | Nealy, Jennifer | 55.90 |
| 3 | Downingtown East | A | 51.84 | ||||
| 4 | West Chester Henderson | A | DQ | ||||
| 5 | Bishop Shanahan | A | DiNardo, Nicoletta | Ranalli, Gretta | Pineda, Kristen | Ebersole, Anna | 53.30 |
| 6 | Bishop Shanahan | C | Brower, Caroline | Shultz, Corinne | Shultz, Cara | Sendi, Caroline | 58.70 |