| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Zielinski, Jake | Great Valley | 53.10 |
| 2 | Newell, Aaron | West Chester Henderson | 53.20 |
| 3 | DeSantis, Brendan | Great Valley | 53.90 |
| 4 | Ruark, Connor | Avon Grove | 53.95 |
| 5 | Dixon, Jacob | Downingtown West | 54.00 |
| 6 | Dever, Aiden | Downingtown East | 54.39 |
| 7 | Kozel, Daniel | Avon Grove | 54.50 |
| 8 | Nolan, Pierce | Great Valley | 54.90 |
| 9 | Lennon, Noah | Avon Grove | 55.00 |
| 10 | Conzelmann, JJ | Downingtown West | 55.23 |
| 11 | Bandari, Agasthya | Great Valley | 55.30 |
| 12 | McCullough, Rhyland | Downingtown West | 56.00 |
| 13 | Stigora, Elisha | West Chester Rustin | 56.00 |
| 14 | Boclair, Justin | Great Valley | 56.00 |
| 15 | Lubeski, Preston | West Chester Henderson | 56.04 |
| 16 | Zouras, Dominic | Downingtown West | 56.24 |
| 17 | Binstead, Thaddeus | Avon Grove | 56.50 |
| 18 | Suby, Pranav | Downingtown West | 56.54 |
| 19 | Potter, Will | West Chester Henderson | 56.80 |
| 20 | Vitale, Drake | Great Valley | 56.90 |
| 21 | Nguyen, Ben | Great Valley | 57.40 |
| 22 | Lee, Daniel | Great Valley | 57.60 |
| 23 | DiFonzo, Neil | West Chester East | 58.00 |
| 24 | Otieno, Sam | Unionville | 58.00 |
| 25 | Quinn, Conor | West Chester East | 58.10 |
| 26 | Ferguson, Ryan | West Chester Rustin | 58.50 |
| 27 | Seymour, Elliott | West Chester Rustin | 58.50 |
| 28 | Langan, Aidan | West Chester Henderson | 58.55 |
| 29 | White, Justin | Unionville | 58.57 |
| 30 | Bertram, Ryan | Downingtown West | 58.75 |
| 31 | Bartilomo, Austin | Downingtown West | 58.94 |
| 32 | Ragni, Mitchell | West Chester East | 59.00 |
| 33 | Hjaltson, Simon | Unionville | 59.00 |
| 34 | Johnson, Falls | Great Valley | 59.00 |
| 35 | Hicks, Bobby | West Chester Henderson | 59.22 |
| 36 | DeRosa, Gabriel | West Chester East | 59.24 |
| 37 | DeVries, Cooper | West Chester Henderson | 59.25 |
| 38 | Ocampo, Richard | Avon Grove | 59.99 |
| 39 | Williams, Zavion | Downingtown West | 1:00.00 |
| 40 | Goines, Ashura | West Chester East | 1:00.00 |
| 41 | Zimmerman, Brendan | West Chester East | 1:00.00 |
| 42 | Bergin, Matthew | West Chester East | 1:01.00 |
| 43 | Burns, William | Unionville | 1:01.00 |
| 44 | Pron, Carson | West Chester Henderson | 1:01.55 |
| 45 | Barton, Tyler | Avon Grove | 1:01.73 |
| 46 | Facciolo, Angelo | Downingtown West | 1:02.00 |
| 47 | Sen, Indraneil | Great Valley | 1:02.40 |
| 48 | Teich, Max | Downingtown West | 1:03.00 |
| 49 | Heckman, Nicholas | Avon Grove | 1:03.00 |
| 50 | Mudge, Jacob | Unionville | 1:04.00 |
| 51 | Roberts, Niko | Avon Grove | 1:04.00 |
| 52 | Reger, Nathaniel | Avon Grove | 1:04.11 |
| 53 | Bender, Wayne | Unionville | 1:04.66 |
| 54 | Morello, Andrew | Downingtown West | 1:05.00 |
| 55 | Marczely, Joey | Avon Grove | 1:06.00 |
| 56 | Renzi, Michael | West Chester East | 1:08.00 |
| 57 | Grieco, Zachary | Unionville | 1:08.00 |
| 58 | Morrison, Sean | West Chester Henderson | 1:09.30 |
| 59 | Witmer, Jacob | West Chester Henderson | 1:10.78 |
| 60 | Patel, Krish | West Chester East | 1:15.00 |
| 61 | Mandl, Caden | Unionville | 1:20.00 |
| 62 | McDermond, Max | West Chester East | 1:25.24 |