| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Schreiber, Sarah | Downingtown West | 59.80 |
| 2 | Ceulers, Ava | Bishop Shanahan | 1:04.00 |
| 3 | Veenema, Eden | Avon Grove | 1:04.00 |
| 4 | Cousin, Kaylin | Avon Grove | 1:04.02 |
| 5 | Franco, Lauren | West Chester Henderson | 1:04.05 |
| 6 | Henning, Lily | Downingtown West | 1:04.19 |
| 7 | Cristinzio, Stephanie | Great Valley | 1:04.60 |
| 8 | Piccone, Sofia | West Chester Rustin | 1:05.00 |
| 9 | Clark, Paige | Avon Grove | 1:05.40 |
| 10 | Weaver, Grace | West Chester Henderson | 1:05.62 |
| 11 | Schiele, Michaela | West Chester East | 1:06.00 |
| 12 | Delate, Kathleen | Bishop Shanahan | 1:06.00 |
| 13 | Hoffritz, Julia | Great Valley | 1:06.80 |
| 14 | Miller, Olivia | Bishop Shanahan | 1:07.00 |
| 15 | Hoffman, Hilary | West Chester Henderson | 1:07.36 |
| 16 | Chylack, Carlie | Downingtown West | 1:07.55 |
| 17 | Greulich, Marguerite | West Chester East | 1:08.00 |
| 18 | Moneme, Chinonye | West Chester East | 1:08.00 |
| 19 | Conord, Paris | Unionville | 1:08.00 |
| 20 | Ciuffetelli, Eva | Avon Grove | 1:08.00 |
| 21 | Driscoll, Delaney | Avon Grove | 1:08.19 |
| 22 | Compher, Emma | Downingtown West | 1:08.50 |
| 23 | Uhlman, Deborah | Bishop Shanahan | 1:09.00 |
| 24 | Sweet, Caitlyn | Avon Grove | 1:09.06 |
| 25 | Hufnagel, Aubrey | Downingtown West | 1:09.33 |
| 26 | Keefer, Ellie | West Chester Rustin | 1:09.55 |
| 27 | McKeown, Kelli | Great Valley | 1:09.70 |
| 28 | Marasco, Julia | Downingtown West | 1:10.00 |
| 29 | Driscoll, Addison | Avon Grove | 1:11.00 |
| 30 | Giaquinto, Kaylee | West Chester Henderson | 1:11.44 |
| 31 | Rogelstad, Leah | Downingtown West | 1:11.67 |
| 32 | Zajac, Gianna | West Chester Rustin | 1:11.71 |
| 33 | Fuhr, Mya | Downingtown West | 1:12.00 |
| 34 | Gatland, Jillian | Downingtown West | 1:12.00 |
| 35 | Montgomery, Alexis | Downingtown West | 1:12.00 |
| 36 | Slate, Rylee | Downingtown West | 1:12.00 |
| 37 | Jarman, Calista | Downingtown West | 1:12.24 |
| 38 | Donohue, Drew | Unionville | 1:13.00 |
| 39 | Wu, Cherry | Unionville | 1:13.00 |
| 40 | Finkbiner, Ashley | Downingtown West | 1:14.00 |
| 41 | Nachani, Salma | Great Valley | 1:14.50 |
| 42 | Schelp, Acacia | Unionville | 1:15.00 |
| 43 | Martchek, Hailey | Downingtown West | 1:15.40 |
| 44 | Proietti, Sarah | Downingtown West | 1:17.98 |
| 45 | Rineer, Emily | Avon Grove | 1:20.00 |
| 46 | VanDyke, Maggie | West Chester East | 1:20.93 |
| 47 | Mittal, Anshi | West Chester East | 1:21.00 |
| 48 | Jimenez, Natalia | West Chester East | 1:30.00 |
| 49 | Alston, Jada | Downingtown West | NT |
| 50 | Hangey, Rebecca | Bishop Shanahan | NT |