| # | Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|---|
| 1 | 15 | Jaisingh, James | 15 | Unattached | 47.77 |
| 2 | 36 | Rossi, Tyler | 14 | Unattached | 51.00 |
| 3 | 12 | Colon, Juan | JR | Unattached | 52.40 |
| 4 | 111 | Ezell, London | SO | PA United | 53.00 |
| 5 | 30 | Marshall, Isaiah | 13 | Unattached | 53.00 |
| 6 | 1 | Fofana, Abdubakar | SR | Dobbins Tech | 54.98 |
| 7 | 101 | Smith, Evan | JR | PA United | 55.54 |
| 8 | 67 | Henle, Alexander | SO | West Chester Track Club | 55.80 |
| 9 | 37 | Vaughn, Frederick | 9 | Unattached | 55.82 |
| 10 | 34 | Harlow, Rylan | 8 | Unattached | 55.83 |
| 11 | 33 | Dinkins, Delvin | 47 | Unattached | 56.00 |
| 12 | 116 | Subratie, Aniff | Unattached | 56.78 | |
| 13 | 31 | Poole, Dustin | 29 | Unattached | 59.00 |
| 14 | 13 | Hipple, Mason | 9 | Unattached | 59.41 |
| 15 | 113 | Staller, Aiden | 9 | PA United | 1:04.74 |
| 16 | 19 | Littlejohn, Kennedi | 6 | Unattached | 1:07.60 |
| 17 | 76 | Riddick, Jasmine | 8 | West Chester Track Club | 1:15.00 |
| 18 | 6 | Anderson, Heylan | SR | Unattached | 1:19.10 |
| 19 | 35 | Marano, Kristin | SR | Unattached | 1:26.00 |
| 20 | 109 | Moseley, Maisie | 3 | PA United | 1:35.10 |
| 21 | 16 | Kellojor, Xaiden | 2 | Unattached | 1:45.00 |
| 22 | 17 | Kellojor, Xaiver | 2 | Unattached | 1:45.00 |
| 23 | 112 | Nagarajan, Yuvathi | 6 | PA United | NT |
| 24 | 110 | Tate, Liam | 4 | PA United | NT |
| 25 | 108 | Marcucio, Rylan | 3 | PA United | NT |
| 26 | 80 | Walker, Grace | 3 | West Chester Track Club | NT |