| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Brooks, Maddy | Bishop McDevitt | 44.84 |
| 2 | Ehrenhalt, Amanda | Penn Charter | 46.13 |
| 3 | Matthews, Jasmine | Springside Chestnut Hill Academy | 47.24 |
| 4 | O'Brien, Shannon | Methacton | 47.25 |
| 5 | Winfield, Mariah | Cheltenham | 47.32 |
| 6 | Lapp, Grace | Conestoga Valley | 47.44 |
| 7 | O'Donnell, Kenslee | Methacton | 47.50 |
| 8 | Donovan, Arianna | St. Mark's High School | 48.34 |
| 9 | Becker, Katie | Warwick | 48.34 |
| 10 | Owens, Mackenzie | Perkiomen Valley | 48.43 |
| 11 | Garozzo, Gianna | Washington Twp HS | 48.50 |
| 12 | Marechal-Raymond, Juliania | Springside Chestnut Hill Academy | 48.76 |
| 13 | Price, Miracle | Friends' Central | 48.79 |
| 14 | Raczkowski, Kalee | Lake Lehman | 49.36 |
| 15 | Steere, Camryn | Methacton | 49.46 |
| 16 | Johnson, Ella | Washington Twp HS | 49.50 |
| 17 | Keegan, Molly | Methacton | 49.58 |
| 18 | Mariano, Alayna | St. Mark's High School | 49.60 |
| 19 | Jones, Dakota | Washington Twp HS | 49.70 |
| 20 | Henriquez, Madison | Bay Shore | 50.00 |
| 21 | Gargan, Riley | Bay Shore | 50.00 |
| 22 | Rodgers, Talia | Abington | 50.10 |
| 23 | Croiger, Kayla | Spring Ford | 50.16 |
| 24 | Gerhart, Isabelle | Methacton | 50.21 |
| 25 | Nolan, Zoe | West Chester Henderson | 50.26 |
| 26 | Roland, Olivia | Penn Charter | 50.30 |
| 27 | Miller, Sara | Eastern Lebanon County | 50.38 |
| 28 | Koenig, Jess | Conestoga Valley | 50.44 |
| 29 | Tutt, Annalie | Dallastown | 50.45 |
| 30 | Reihm, Thalia | Northeastern | 50.47 |
| 31 | Compher, Emma | Downingtown West | 50.62 |
| 32 | Harris, Darian | Tatnall | 51.01 |
| 33 | Fuhr, Mya | Downingtown West | 51.68 |
| 34 | Johnson, Camille | Tatnall | 53.51 |
| 35 | Sujesh, Keerthana | Downingtown East | NT |
| 36 | Lentz, Veronica | Penn Charter | NT |