| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Spring Ford | A | 49.42 | ||||
| 2 | Wilson (3) | A | 49.59 | ||||
| 3 | Washington Twp HS | A | Nesmith | Beasley | Hetzel | Giloley | 49.59 |
| 4 | Methacton | A | 49.87 | ||||
| 5 | Downingtown East | A | Binns | Fugua | Jennings-Scott | Riley | 49.97 |
| 6 | Coatesville | A | 50.05 | ||||
| 7 | Conestoga Valley | A | Schnapf | Johnson | Montijo-Ortiz | Linder | 50.37 |
| 8 | Souderton Area | A | Schulingkamp | Pettaway | Krebs | Mills | 50.43 |
| 9 | Owen J. Roberts | A | Bold | Larsen | McGuire | Weaver | 50.46 |
| 10 | Unionville | A | 50.51 | ||||
| 11 | Abington | A | Smith | Woodridge | Taylor | Black | 51.68 |
| 12 | Northeastern | A | Brown | Calder | Davis | Herod | 51.72 |
| 13 | Bishop Shanahan | A | Hangey | Wolfe | Ceulers | Ceulers | 52.44 |
| 14 | Penn Charter | A | 52.47 | ||||
| 15 | Upper Merion | A | 53.00 | ||||
| 16 | Perkiomen Valley | A | 53.50 | ||||
| 17 | William Penn | A | 53.50 | ||||
| 18 | Bay Shore | A | Shelton | Mann | Rodgers | Snell | 54.00 |
| 19 | West Chester Henderson | A | 54.23 |