| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Clarke, Allyson | WC East | 1:04.00 |
| 2 | Mucha, Grace | D-West | 1:04.10 |
| 3 | Franco, Lauren | Henderson | 1:04.40 |
| 4 | Schiele, Michaela | WC East | 1:05.00 |
| 5 | Flannery, Lauren | D-West | 1:05.75 |
| 6 | Stanfield, Chloe | D-West | 1:05.78 |
| 7 | Keefer, Ellie | Rustin | 1:07.00 |
| 8 | McClain, Alexys | Rustin | 1:07.00 |
| 9 | Quinn, Maegan | Rustin | 1:07.00 |
| 10 | Rekito, Hannah | Rustin | 1:07.00 |
| 11 | Fernandes, Gabi | Henderson | 1:07.64 |
| 12 | Miller, Olivia | Bishop Shanahan | 1:07.78 |
| 13 | Greulich, Marguerite | WC East | 1:08.00 |
| 14 | Alvarez, Kimberly | Kennett | 1:08.50 |
| 15 | Main, Molly | Unionville | 1:09.00 |
| 16 | Fetrow, Leksie | Oxford Area | 1:09.31 |
| 17 | Bryan, Autumn | Henderson | 1:09.60 |
| 18 | Relacion, Angelica | Bishop Shanahan | 1:10.59 |
| 19 | Cicconi, Carly | Kennett | 1:11.50 |
| 20 | Daly, Caroline | Bishop Shanahan | 1:12.28 |
| 21 | Wolfe, Elizabeth | Bishop Shanahan | 1:14.17 |
| 22 | Ward, Lindsey | Bishop Shanahan | 1:14.27 |
| 23 | Foret, Ella | Bishop Shanahan | 1:15.00 |
| 24 | Illich, Mara | Rustin | 1:16.00 |
| 25 | Hangey, Rebecca | Bishop Shanahan | 1:17.00 |
| 26 | Ward, Kirsten | Bishop Shanahan | 1:17.90 |
| 27 | Williams, Rose | Bishop Shanahan | 1:18.35 |
| 28 | Uhlman, Deborah | Bishop Shanahan | 1:19.00 |
| 29 | Wallace, Amanda | Bishop Shanahan | 1:19.00 |
| 30 | Klein, Noelle | Bishop Shanahan | 1:20.00 |
| 31 | Wilmer, Gretchen | Bishop Shanahan | 1:20.00 |
| 32 | McGrory, Megan | Bishop Shanahan | 1:23.00 |
| 33 | Hewitt, Evelyn | Bishop Shanahan | 1:24.00 |
| 34 | Chan, Abbey | Bishop Shanahan | 1:27.86 |
| 35 | Lane, Grace | Bishop Shanahan | 1:28.00 |
| 36 | Keenan, Ella | Bishop Shanahan | 1:28.21 |
| 37 | Carr, Caitlin | Bishop Shanahan | 1:30.00 |