| # | Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|---|
| 1 | 503 | Elliott, Avery | SR | Episcopal Academy | 45.41 |
| 2 | 907 | Drumwright, Payton | JR | Engineering & Science | 45.50 |
| 3 | 859 | Mobley, Penelope | SR | Academy at Palumbo | 47.04 |
| 4 | 1146 | Natitus, Julia | FR | Dallas | 48.0h |
| 5 | 32 | Outland, Kennedy | SR | Haverford Township | 48.10 |
| 6 | 1249 | Wagner, Colette | Haverford Township | 48.50 | |
| 7 | 49 | Kraus, Kacey | SO | Haverford Township | 48.51 |
| 8 | 678 | Alli-Kelly, Kalin | SO | Springfield (Delco) | 49.15 |
| 9 | 912 | Massey, Rayana | JR | Engineering & Science | 49.17 |
| 10 | 319 | Tomassetti, Elizabeth | JR | Msgr Bonner & Abp Prendergast | 49.20 |
| 11 | 116 | Keares, Mariel | JR | Manheim Township | 49.94 |
| 12 | 1182 | Grimes, Katelyn | JR | Great Valley | 50.47 |
| 13 | 707 | Harshyne, Ella | SR | Archmere Academy | 50.50 |
| 14 | 1136 | Keller, Kaitlyn | JR | Dallas | 50.56 |
| 15 | 1014 | Huang, Janet | SO | Strath Haven | 50.71 |
| 16 | 70 | Kelley, Maddy | SR | Villa Maria | 50.98 |
| 17 | 1250 | Abreau, Eva | Haverford Township | 51.20 | |
| 18 | 1011 | Cole, Ellie | SO | Strath Haven | 51.90 |
| 19 | 839 | Chearney, Makenzie | FR | Western Wayne | 51.9h |
| 20 | 723 | Harris, Marlise | FR | Archmere Academy | 52.2h |
| 21 | 822 | Gennarelli, Katherine | FR | Conestoga | 52.29 |
| 22 | 932 | Kaba, Fatumata | SR | Academy Park | 52.43 |
| 23 | 591 | Salley, Makhiya | JR | Agnes Irwin | 53.45 |
| 24 | 464 | Karboski, Veronica | JR | Emmaus | 53.75 |
| 25 | 474 | Truckenmiller, Adelynn | SO | Emmaus | 53.8h |
| 26 | 104 | Shorter, Sofia | JR | Manheim Township | 54.01 |
| 27 | 813 | Sudhagar, Rheeya | SO | Conestoga | 54.04 |
| 28 | 187 | Turay, Auldine | SO | Ridley | 54.10 |
| 29 | 209 | Pratt, Tessa | JR | Villa Joseph Marie | 54.87 |
| 30 | 268 | McNamee, Julia | SO | Phoenixville Area | 55.20 |
| 31 | 1038 | Pugliese, Katherine | SO | Lower Merion | 56.00 |
| 32 | 679 | Hayden, Danayah | SO | Springfield (Delco) | 56.15 |
| 33 | 270 | Smith, Luci | JR | Phoenixville Area | 56.93 |
| 34 | 1072 | Tankhilevich, Mila | SO | Lower Moreland | 57.00 |
| 35 | 161 | Gensemer, Madison | JR | Ridley | 57.06 |
| 36 | 20 | Lengel, Olivia | SO | Quakertown | 57.62 |
| 37 | 869 | Daughtry, Lani | SO | Academy at Palumbo | 58.0h |
| 38 | 569 | Sanders, Marissa | JR | Crestwood | 58.0h |
| 39 | 80 | Dunn, Natalie | SO | Villa Maria | 58.00 |
| 40 | 573 | Eppley, Cieraena | SR | Crestwood | 58.0h |
| 41 | 243 | Lakofsky, Caitlyn | SO | West Chester Rustin | 58.00 |
| 42 | 556 | Cooper, Nahla | SO | Radnor | 58.55 |
| 43 | 410 | Rose, Layah | FR | Norristown | 58.92 |
| 44 | 933 | Andrews, Samiiya | SR | Academy Park | 1:00.49 |
| 45 | 1061 | Avington, Ashley | JR | Lower Moreland | 1:01.0h |
| 46 | 1183 | Lee, Kate | JR | Great Valley | NT |