| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | Pajrowski, Jonathon | SR | Unattached | 4:58.56 |
| 2 | Spronk, Brandon | 9 | Unattached | 5:00.00 |
| 3 | Sargent, Brendan | SO | Unattached | 5:00.00 |
| 4 | Beels, Marten | 39 | Unattached | 5:01.00 |
| 5 | Watson, Greg | 53 | Unattached | 5:30.00 |
| 6 | March, Megan | 33 | Unattached | 5:45.00 |
| 7 | Trinidad, Cristian | 13 | Rainbow Rabbits | 5:48.00 |
| 9 | Bahnatka, Lauren | 35 | Eagleville Elite | 6:04.74 |
| 10 | Carlin, Sami | 32 | Unattached | 6:20.00 |
| 11 | Roberts, Sarah | 33 | Unattached | 6:50.00 |