| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | Robson, Clare | D-East | 2:26.31 |
| 2 | Shanmugasundaram, Lalitha | D-West | 2:36.44 |
| 3 | Barfell, Brooke | Avon Grove | 2:40.74 |
| 4 | Sandercock, Kyra | D-East | 2:35.59 |
| 5 | Riccardo, Ciana | D-West | 2:37.76 |
| 6 | Allison, Maeve | Avon Grove | 2:41.31 |
| 7 | McDermott, Mallory | D-East | 2:40.13 |
| 8 | Datte, Tessa | D-West | 2:39.79 |
| 9 | Chandler, Addison | Avon Grove | 2:44.57 |
| 10 | Jackson, Lauren | D-East | 2:41.32 |
| 11 | Proietti, Elizabeth | D-West | 2:44.32 |
| 12 | Shaw, Savannah | D-West | 2:41.11 |
| 13 | Hibshman, Livia | D-East | 2:45.48 |
| 14 | McGinty, Hannah | D-East | 2:45.76 |
| 15 | Leadbeater, Jenna | D-East | 2:56.63 |
| 16 | Peck, Kari | D-West | 3:04.11 |
| 17 | Corporal, Emily | D-East | 3:12.81 |
| 18 | Dyas, Emma | D-West | 3:13.32 |
| 19 | Defibaugh, Stella | D-West | 3:13.67 |
| 20 | Welsh, Hailey | D-West | 3:30.22 |
| 21 | Hernandez, Alessandra | D-West | 3:33.57 |
| 22 | Cline, Maisy | D-West | NT |