| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Stanfield, Chloe | D-West | 48.78 |
| 2 | Pfaff, Annika | Avon Grove | 50.55 |
| 3 | Smith, Payton | Avon Grove | 51.89 |
| 4 | Ericson, Rylee | D-East | 52.44 |
| 5 | Davis, Kelly | D-West | 53.14 |
| 6 | Roberts, Mary | Avon Grove | 55.11 |
| 7 | Baumann, Chloe | D-East | 55.44 |
| 8 | Minchoff, Lucy | D-West | 56.95 |
| 9 | Shaefer, Reilly | D-East | 1:00.00 |
| 10 | Shaw, Charlotte | D-East | 1:00.28 |
| 11 | Hauer, Carly | D-East | 1:00.40 |
| 12 | McKeon, Marisa | D-East | 1:04.53 |
| 13 | Wise, Ava | D-East | 1:09.35 |
| 14 | Swinton, Allison | D-East | 1:10.00 |
| 15 | Navani, Vanshee | D-East | 1:14.16 |
| 16 | Kelch, Nikki | D-East | 1:14.24 |
| 17 | Penmetsa, Mahitha | D-East | NT |