| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Alexander, Courtney | West Chester Henderson | 5:22.18 |
| 2 | Miller, Sammie | Coatesville | 5:30.00 |
| 3 | Kitchen, Courtney | West Chester Henderson | 5:35.36 |
| 4 | Rivituso, Kristin | West Chester Henderson | 5:54.14 |
| 5 | Omiridis, Marissa | West Chester Henderson | 6:05.00 |
| 6 | Clark, Carly | Avon Grove | 6:05.00 |
| 7 | Ng, Chloe | West Chester Henderson | 6:09.08 |
| 8 | Ackerman, Katie | West Chester Henderson | 6:16.00 |
| 9 | Campbell, Kyla | West Chester Henderson | 6:19.77 |
| 10 | Talley, Peyton | West Chester Henderson | 6:20.00 |
| 11 | Cuesta, Julia | West Chester Henderson | 6:22.50 |
| 12 | Shah, Jaini | West Chester Henderson | 6:23.00 |
| 13 | Paglia, Stephanie | Avon Grove | 6:26.93 |
| 14 | Blakely, Olivia | West Chester Henderson | 6:33.00 |
| 15 | Russell, Molly | Avon Grove | 6:34.20 |
| 16 | Forward, Kathy | Coatesville | 6:34.42 |
| 17 | Zoretic, Olivia | West Chester Henderson | 6:35.00 |
| 18 | Zheng, Alison | West Chester Henderson | 6:40.00 |
| 19 | Watson, Sophia | West Chester Henderson | 6:45.00 |
| 20 | Marion, Kate | West Chester Henderson | 6:50.00 |
| 21 | Barfell, Brooke | Avon Grove | NT |
| 22 | Chandler, Addison | Avon Grove | NT |
| 23 | Crist, Isabella | Avon Grove | NT |
| 24 | Smith, Jessica | Avon Grove | NT |