| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Pfaff, Annika | Avon Grove | 48.00 |
| 2 | Eberly, Arianna | Coatesville | 48.76 |
| 3 | Smith, Payton | Avon Grove | 51.00 |
| 4 | Roberts, Mary | Avon Grove | 53.00 |
| 5 | Desrosiers, Bailey | West Chester Henderson | 54.00 |
| 6 | Diguiseppe, Emma | West Chester Henderson | 55.00 |
| 7 | Gemmell, Rachel | Coatesville | 56.97 |
| 8 | Davis, Xianna | Coatesville | 57.34 |
| 9 | Hill, Megan | West Chester Henderson | 58.70 |
| 10 | Fedison, Haley | Coatesville | 1:04.96 |