| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Brown, Madison | D-East | 5:26.87 |
| 2 | Steinberg, Emily | D-East | 5:28.74 |
| 3 | Segrave-Daly, Carlyn | Bishop Shanahan | 5:31.53 |
| 4 | Pfaff, Annika | Avon Grove | 5:32.73 |
| 5 | Willis, Meghan | Bishop Shanahan | 5:34.85 |
| 6 | Pfaff, Ava | Avon Grove | 5:34.90 |
| 7 | Myers, Ava | D-East | 5:41.00 |
| 8 | Lacey, Jenna | D-East | 5:41.33 |
| 9 | Witkowski, Ava | D-West | 5:41.90 |
| 10 | Olsen, Lucy | WC Henderson | 5:42.03 |
| 11 | Kelly, Amanda | WC Henderson | 5:42.12 |
| 12 | Bender, Riley | WC Henderson | 5:43.72 |
| 13 | Ward, Jessica | Bishop Shanahan | 5:46.43 |
| 14 | Tyner, Alex | D-West | 5:48.75 |
| 15 | Taylor, Melanie | D-East | 5:49.88 |
| 16 | Hufnagel, Aubrey | D-West | 5:50.22 |
| 17 | Dortone, Riley | D-West | 5:50.84 |
| 18 | Kaas, Elsie | Bishop Shanahan | 5:52.95 |
| 19 | Diebold, Oriah | D-West | 5:53.95 |
| 20 | Moser, Keira | D-East | 5:55.35 |
| 21 | Heebner, Maria | WC East | 6:00.24 |
| 22 | Schindler, Lily | Avon Grove | 6:00.55 |
| 23 | Corsi-Martens, Emma | D-East | 6:01.93 |
| 24 | Robinson, Cailyn | D-West | 6:02.05 |
| 25 | Rymer, Bridget | D-West | 6:02.71 |
| 26 | Moser, Jocelyn | D-East | 6:03.50 |
| 27 | Guidotti, Hannah | WC Henderson | 6:04.49 |
| 28 | Chermak, Carly | WC East | 6:05.18 |
| 29 | Sciulli, Lauren | D-West | 6:05.73 |
| 30 | Mills, Emma | Avon Grove | 6:05.94 |
| 31 | Bennett, Julia | Bishop Shanahan | 6:06.13 |
| 32 | Terry, Allison | D-East | 6:13.57 |
| 33 | Smyth, Julia | Bishop Shanahan | 6:14.42 |
| 34 | Loeper, Penelope | Bishop Shanahan | 6:15.00 |
| 35 | Nazareth, Maryann | D-East | 6:18.17 |
| 36 | McCaffrey, Lily | WC East | 6:18.75 |
| 37 | Stypulkowski, Lauren | WC East | 6:19.24 |
| 38 | Burkhardt, Catherine | Bishop Shanahan | 6:21.77 |
| 39 | Hewitt, Evelyn | Bishop Shanahan | 6:22.38 |
| 40 | Ryan, Molly | Avon Grove | 6:23.02 |
| 41 | VanDyke, Maggie | WC East | 6:25.30 |
| 42 | O'Brien, Grace | Coatesville | 6:28.34 |
| 43 | Vaughen, Kate | D-West | 6:28.58 |
| 44 | Omiridis, Marissa | WC Henderson | 6:29.46 |
| 45 | Buffington, Fiona | Avon Grove | 6:30.20 |
| 46 | Davidson, Emma | WC Henderson | 6:30.24 |
| 47 | Foster, Bella | WC Henderson | 6:34.85 |
| 48 | Godin, Eve | WC Henderson | 6:35.00 |
| 49 | Cohen, Maddox | WC Henderson | 6:36.88 |
| 50 | Klein, Noelle | Bishop Shanahan | 6:39.30 |
| 51 | Jensen, Morgan | D-West | 6:40.18 |
| 52 | Colson, Paige | WC Henderson | 6:43.24 |
| 53 | Holman, Ainsley | Avon Grove | 6:54.25 |