| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Avon Grove | A | Keegan | Veenema | Conlan | Smith | 4:05.00 |
| 2 | Downingtown East | A | Binns | Dever | Massey | Mukalian | 4:13.43 |
| 3 | West Chester East | A | Cooper | Leeds | Piombino | Moneme | 4:20.10 |
| 4 | Bishop Shanahan | A | Kyle | Miller | Hangey | McGonagle | 4:25.00 |
| 5 | Downingtown West | A | Fuhr | Marasco | Ramsey | Shearer | 4:30.00 |
| 6 | West Chester Henderson | A | Noble | Noce | Ziemniak | Gordon | 4:36.50 |
| 7 | Bishop Shanahan | B | Mineweaser | Hewitt | Williams | Segrave-Daly | 4:40.00 |
| 8 | Coatesville | A | 4:42.43 | ||||
| 9 | Downingtown West | B | Whelan | Diebold | Dortone | Rogelstad | 4:45.00 |
| 10 | Avon Grove | F | Buening | Caprarola | Etschman | Guizzetti | 4:58.03 |
| 11 | Avon Grove | D | Caulder | Schottin | Ciuffetelli | Ruiz | 4:58.03 |
| 12 | Avon Grove | G | Estrada | Rineer | 4:58.03 | ||
| 13 | Avon Grove | E | Frantz | Oswald | Walker | Molnar | 4:58.03 |
| 14 | Avon Grove | H | Hopkins | McCann | McKnight | Walker | 4:58.03 |
| 15 | Downingtown West | C | Jensen | Hollinger | Reeves | Naik | 5:00.00 |
| 16 | WC East | A | NT | ||||
| 17 | WC East | B | NT | ||||
| 18 | WC Henderson | A | NT | ||||
| 19 | WC Henderson | A | NT | ||||
| 20 | WC Henderson | A | NT | ||||
| 21 | D-West | A | NT | ||||
| 22 | D-West | C | NT | ||||
| 23 | D-West | B | NT | ||||
| 24 | D-West | A | NT | ||||
| 25 | D-West | C | NT | ||||
| 26 | D-West | B | NT | ||||
| 27 | D-West | A | NT | ||||
| 28 | D-West | C | NT | ||||
| 29 | D-West | B | NT | ||||
| 30 | D-East | A | NT | ||||
| 31 | D-East | A | NT | ||||
| 32 | D-East | A | NT |