| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Hearn, Ryleigh | Downingtown West | 25.69 |
| 2 | Binns, Olivia | Downingtown East | 25.91 |
| 3 | Alston, Jada | Downingtown West | 26.03 |
| 4 | Conlan, Leia | Avon Grove | 26.25 |
| 5 | Moneme, Chinonye | West Chester East | 26.25 |
| 6 | Domurat, Chloe | Coatesville | 26.72 |
| 7 | Fugua, Faith | Downingtown East | 26.99 |
| 8 | Keegan, Bridget | Avon Grove | 27.50 |
| 9 | Leeds, Giovina | West Chester East | 27.50 |
| 10 | Piombino, Amelia | West Chester East | 27.66 |
| 11 | Henning, Lily | Downingtown West | 27.70 |
| 12 | Massey, Nia | Downingtown East | 27.77 |
| 13 | Reed, Lauren | Coatesville | 27.79 |
| 14 | Driscoll, Delaney | Avon Grove | 27.85 |
| 15 | Grier, LeAnn | Coatesville | 27.85 |
| 16 | Dever, Olivia | Downingtown East | 27.98 |
| 17 | Ceulers, Ava | Bishop Shanahan | 28.00 |
| 18 | Kuhn, Reilly | Downingtown East | 28.08 |
| 19 | Gardner, Lianna | Avon Grove | 28.09 |
| 20 | Marasco, Julia | Downingtown West | 28.17 |
| 21 | Compher, Emma | Downingtown West | 28.36 |
| 22 | Heckman, Olivia | Avon Grove | 28.40 |
| 23 | Ramsey, Ellen | Downingtown West | 28.65 |
| 24 | Chylack, Carlie | Downingtown West | 28.73 |
| 25 | Caronia, Alex | West Chester Henderson | 28.75 |
| 26 | Shearer, Cecilia | Downingtown West | 28.77 |
| 27 | Hangey, Rebecca | Bishop Shanahan | 28.83 |
| 28 | Noble, Brianna | West Chester Henderson | 28.88 |
| 29 | Miller, Olivia | Bishop Shanahan | 29.09 |
| 30 | Barcalow, Carlene | Downingtown West | 29.21 |
| 31 | Caulder, Zaida | Avon Grove | 29.34 |
| 32 | Wolfe, Elizabeth | Bishop Shanahan | 29.39 |
| 33 | Riley, Katie | Downingtown East | 29.41 |
| 34 | Ashlock, Taylor | West Chester East | 29.43 |
| 35 | Brown, Payton | Avon Grove | 29.50 |
| 36 | Ruiz, Gabriella | Avon Grove | 29.50 |
| 37 | Kurtz, Alena | Avon Grove | 29.50 |
| 38 | Niedzielski, Charlotte | Downingtown East | 29.57 |
| 39 | Fink, Addison | Downingtown East | 29.78 |
| 40 | Cooper, Jada | West Chester East | 29.90 |
| 41 | Noce, Isabella | West Chester Henderson | 29.96 |
| 42 | Ceulers, Sophia | Bishop Shanahan | 29.98 |
| 43 | Scarantino, Gianna | Bishop Shanahan | 30.00 |
| 44 | Figueroa, Adrianna | Coatesville | 30.05 |
| 45 | Mineweaser, Brooklyn | Bishop Shanahan | 30.50 |
| 46 | Stauffer, Carmen | West Chester Henderson | 30.54 |
| 47 | Bowden, Brooke | Bishop Shanahan | 30.63 |
| 48 | Soto-Villafane, Jaylin | Coatesville | 30.69 |
| 49 | Schottin, Bailey | Avon Grove | 30.73 |
| 50 | Gordon, Erin | West Chester Henderson | 30.77 |
| 51 | Richardson, Kerry | West Chester East | 30.92 |
| 52 | Matulewicz, Ella | Bishop Shanahan | 31.50 |
| 53 | Friz, Grace | Bishop Shanahan | 31.50 |
| 54 | Maloni, Sara | West Chester Henderson | 31.68 |
| 55 | Nolan, Ella | West Chester Henderson | 31.70 |
| 56 | Rivera, Jasmine Rose | Coatesville | 32.00 |
| 57 | Kincaid, Jacqueline | West Chester Henderson | 32.20 |
| 58 | Izzi, Ava | West Chester Henderson | 32.26 |
| 59 | Stewart, Ava | Coatesville | 33.00 |
| 60 | Skomorucha, Leah | West Chester Henderson | 33.50 |
| 61 | Fuhr, Mya | Downingtown West | NT |