| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Taylor, Melanie | Downingtown East | 2:21.92 |
| 2 | Brown, Madison | Downingtown East | 2:27.32 |
| 3 | Kyle, Nora | Bishop Shanahan | 2:28.09 |
| 4 | Pfaff, Annika | Avon Grove | 2:30.46 |
| 5 | Ziemniak, Weronica | West Chester Henderson | 2:30.90 |
| 6 | Pfaff, Ava | Avon Grove | 2:31.31 |
| 7 | Witkowski, Ava | Downingtown West | 2:33.17 |
| 8 | Ward, Jessica | Bishop Shanahan | 2:33.88 |
| 9 | Tyner, Alex | Downingtown West | 2:35.00 |
| 10 | Schindler, Lily | Avon Grove | 2:35.00 |
| 11 | Cline, Piper | Downingtown West | 2:36.00 |
| 12 | Morraye, Olivia | Downingtown West | 2:36.66 |
| 13 | Fielding, Hannah | West Chester Henderson | 2:37.00 |
| 14 | Dortone, Riley | Downingtown West | 2:39.00 |
| 15 | Rymer, Bridget | Downingtown West | 2:40.00 |
| 16 | LaVeyra, Adylee | Downingtown East | 2:40.24 |
| 17 | Piombino, Sonia | West Chester East | 2:40.24 |
| 18 | Lacey, Jenna | Downingtown East | 2:41.24 |
| 19 | Heraghty, Niamh | West Chester Henderson | 2:42.20 |
| 20 | McCaffrey, Lily | West Chester East | 2:42.95 |
| 21 | Moser, Jocelyn | Downingtown East | 2:43.24 |
| 22 | Diebold, Oriah | Downingtown West | 2:45.00 |
| 23 | Mills, Emma | Avon Grove | 2:45.79 |
| 24 | Kleckner, Zoe | West Chester Henderson | 2:45.90 |
| 25 | Shinnen, Sky | Downingtown West | 2:46.84 |
| 26 | Hewitt, Evelyn | Bishop Shanahan | 2:47.44 |
| 27 | Chermak, Carly | West Chester East | 2:49.54 |
| 28 | Mukalian, Dinah | Downingtown East | 2:50.24 |
| 29 | Loeher, Mary | Downingtown East | 2:50.24 |
| 30 | O'Brien, Grace | Coatesville | 2:51.12 |
| 31 | Stypulkowski, Lauren | West Chester East | 2:51.31 |
| 32 | Michell, Molly | West Chester Henderson | 2:51.57 |
| 33 | Jadhav, Eesha | West Chester Henderson | 2:52.74 |
| 34 | Vaughen, Kate | Downingtown West | 2:53.00 |
| 35 | Delucca, Andrea | Downingtown East | 2:53.00 |
| 36 | Whelan, Morgan | Downingtown West | 2:53.63 |
| 37 | Klara, Rachel | West Chester Henderson | 2:55.00 |
| 38 | Godin, Eve | West Chester Henderson | 2:55.00 |
| 39 | Williams, Rose | Bishop Shanahan | 2:55.00 |
| 40 | Kolimago, Katherine | Bishop Shanahan | 2:55.00 |
| 41 | Le, Dora | Bishop Shanahan | 2:55.00 |
| 42 | Uhlman, Sarah | Bishop Shanahan | 2:55.00 |
| 43 | Uhlman, Deborah | Bishop Shanahan | 2:55.00 |
| 44 | Carlow, Ana | West Chester Henderson | 2:59.60 |
| 45 | Cuesta, Julia | West Chester Henderson | 3:00.32 |
| 46 | Holman, Ainsley | Avon Grove | 3:02.00 |
| 47 | Flick, Catherine | Bishop Shanahan | 3:05.00 |
| 48 | Lynch, Sylvie | Bishop Shanahan | 3:05.00 |
| 49 | Dynesko, Kathryn | Coatesville | 3:07.71 |
| 50 | Buffington, Fiona | Avon Grove | 3:10.00 |
| 51 | Ryan, Molly | Avon Grove | 3:15.00 |