| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Smith, Payton | Avon Grove | 47.58 |
| 2 | Ciuffetelli, Eva | Avon Grove | 49.88 |
| 3 | Kurtz, Alena | Avon Grove | 50.50 |
| 4 | Sujesh, Keerthana | Downingtown East | 50.75 |
| 5 | Hangey, Rebecca | Bishop Shanahan | 52.24 |
| 6 | Caronia, Alex | West Chester Henderson | 52.50 |
| 7 | Doran, Kira | Coatesville | 52.87 |
| 8 | Keefe, Maeve | West Chester East | 53.15 |
| 9 | Uhlman, Deborah | Bishop Shanahan | 53.62 |
| 10 | Yarnall-Stevenson, Charlotte | Downingtown East | 54.71 |
| 11 | Davies, Grace | Downingtown East | 55.36 |
| 12 | Nolan, Zoe | West Chester Henderson | 56.03 |
| 13 | Schaefer, Reilly | Downingtown East | 56.11 |
| 14 | Rosado, Ciana | Coatesville | 56.41 |
| 15 | Finkbiner, Ashley | Downingtown West | 57.40 |
| 16 | Soto-Villafane, Jaylin | Coatesville | 58.00 |
| 17 | Loeper, Penelope | Bishop Shanahan | 58.50 |
| 18 | Skomorucha, Leah | West Chester Henderson | 1:00.56 |
| 19 | Klara, Olivia | West Chester Henderson | 1:00.81 |
| 20 | Grabski, Julia | Downingtown East | 1:01.00 |
| 21 | Manuel, Kaila | Downingtown East | 1:01.35 |
| 22 | Guru, Bhavya | Downingtown East | 1:02.00 |
| 23 | Rogelstad, Sarah | Downingtown West | 1:02.14 |
| 24 | Robinson, Julia | Downingtown East | 1:02.61 |
| 25 | Lueders, Mackenzie | Downingtown East | 1:02.67 |
| 26 | Gray, Leah | Avon Grove | 1:03.32 |
| 27 | Brazunas, Mckenna | Downingtown East | NT |