| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Ford, Elijah | D-West | 49.02 |
| 2 | Stocker, Jabarri | Coatesville | 50.06 |
| 3 | Lubeski, Preston | WC Henderson | 50.17 |
| 4 | Dixon, Jacob | D-West | 50.69 |
| 5 | Kelly, Dave | Coatesville | 50.79 |
| 6 | Miller, Joshua | Coatesville | 50.90 |
| 7 | Nakahara, Will | D-East | 51.10 |
| 8 | Kopanski, Sean | D-East | 52.01 |
| 9 | Moyer, Sam | D-East | 52.21 |
| 10 | Allen, Nathan | Avon Grove | 52.43 |
| 11 | Silvester, Tommy | Coatesville | 52.45 |
| 12 | Dever, Aidan | D-East | 53.24 |
| 13 | Wykie, Ben | D-East | 53.36 |
| 14 | McGinn, Gavin | Avon Grove | 53.70 |
| 15 | Shirker, Adam | Bishop Shanahan | 53.91 |
| 16 | Hibshman, Owen | D-East | 54.21 |
| 17 | Harper, Marcel | Coatesville | 54.32 |
| 18 | Bell, Kelly | D-West | 54.65 |
| 19 | Shannon, Aiden | Bishop Shanahan | 54.98 |
| 20 | Barton, Tyler | Avon Grove | 55.11 |
| 21 | Basvapatri, Srihan | WC East | 55.45 |
| 22 | Green, Sincere | Coatesville | 55.50 |
| 23 | Polizzi, Jack | Bishop Shanahan | 55.60 |
| 24 | Murugan, Karthik | D-East | 55.88 |
| 25 | Gibbons, Tommy | D-East | 55.88 |
| 26 | Todd, Jameson | Bishop Shanahan | 55.98 |
| 27 | Shappell Smith, Simeon | WC Henderson | 55.99 |
| 28 | Taraba, Ben | Avon Grove | 56.00 |
| 29 | Watson, Damon | Coatesville | 56.32 |
| 30 | Goodwin, Landon | WC East | 56.50 |
| 31 | McCaffrey, Stephen | WC East | 56.50 |
| 32 | Okafur, CJ | Bishop Shanahan | 56.50 |
| 33 | Alex, Kam | D-West | 56.84 |
| 34 | Snyder, Patrick | WC Henderson | 56.86 |
| 35 | Morton, Albert | Coatesville | 56.95 |
| 36 | Bergin, Matthew | WC East | 57.00 |
| 37 | Conte, Samuel | Bishop Shanahan | 57.25 |
| 38 | Whitaker, Sean | Bishop Shanahan | 57.87 |
| 39 | Brice, Jake | WC Henderson | 57.92 |
| 40 | Shank, Mason | D-West | 57.92 |
| 41 | Casner, Zaire | D-West | 58.03 |
| 42 | Roberts, Niko | Avon Grove | 58.09 |
| 43 | Mosquera, Thomas | Bishop Shanahan | 58.15 |
| 44 | Balick, Sam | Avon Grove | 58.18 |
| 45 | Baum, Alex | WC Henderson | 58.37 |
| 46 | Billig, James | Avon Grove | 58.50 |
| 47 | Oliver, Gavin | D-West | 58.52 |
| 48 | Smart, Clayton | D-West | 58.78 |
| 49 | Taylor, Gavin | D-East | 58.80 |
| 50 | Bezawada, Tanish | D-East | 58.83 |
| 51 | Sheridan, Damian | WC East | 59.00 |
| 52 | Benner, Dylan | D-West | 59.68 |
| 53 | Kurdziel, Evan | WC Henderson | 59.87 |
| 54 | Rawa, William | WC Henderson | 59.92 |
| 55 | Clark, Tyler | Avon Grove | 59.99 |
| 56 | Daller, DJ | Coatesville | 1:00.05 |
| 57 | Filipas, Mike | WC Henderson | 1:00.31 |
| 58 | Facciolo, Angelo | D-West | 1:00.33 |
| 59 | Kulp, Fenton | WC East | 1:00.59 |
| 60 | Gregory, Eric | Avon Grove | 1:00.83 |
| 61 | Goines, Ashura | WC East | 1:01.00 |
| 62 | Deitrich, Robert | Avon Grove | 1:01.00 |
| 63 | Al-Tikriti, Becker | WC East | 1:02.00 |
| 64 | Pentz, Ben | WC Henderson | 1:02.20 |
| 65 | Baynard, Qunicy | Coatesville | 1:03.72 |
| 66 | Richards, Owen | WC Henderson | 1:05.01 |